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Human CD27 Protein, mFc-His Tag

Recombinant human CD27 protein with C-terminal mouse Fc and 6XHis tag


Cat.#:  11141

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11141
Product Name:   Human CD27 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD27;TNFRSF7;S152;T14;Tp55
Target:    CD27
UNIPROT ID:    P26842
Description:    Recombinant human CD27 protein with C-terminal mouse Fc and 6xHis tag
Background:     The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 65-72 kDa after removal of the signal peptide.
Molecular Characterization:    CD27(Ala20-Arg191) mFc(Pro99-Lys330) 6×His
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD27 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD27, mFc-His tagged protein (11141) can bind Anti-CD27 Neutralizing antibody 28027 in a linear range of 0.64-16.0 ng/ml.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD27, mFc-His tagged protein  (11141) can bind Human CD70,hFc-His tagged protein 11294 in a linear range of 3.2-80 ng/ml.

Figure 4. Flow cytometry analysis with 15 µg/ml Human CD27 Protein, mFc-His tag (11141) on Expi293 cells transfected with human CD70 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).