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Human CD28 Protein, mFc-His Tag

Recombinant human CD28 protein with C-terminal mouse Fc and 6XHis tag
5/5

$555.00

Cat.#:  11142

   Size:   100 μg

In Stock

            Product Description          
Cat.#:     11142
Product Name:   Human CD28 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD28;Tp44
Target:    CD28
UNIPROT ID:    P10747
Description:    Recombinant human CD28 protein with C-terminal mouse Fc and 6xHis tag
Background:     The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 68-72 kDa after removal of the signal peptide.
Molecular Characterization:    CD28(Asn19-Pro152) mFc(Pro99-Lys330) 6×His
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD28 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-1, hFc tagged protein 11283 can bind Human CD28, mFc-His tagged protein (11142) in a linear range of 125-4000 ng/ml.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human B7-2, hFc tagged protein 11284 can bind Human CD28, mFc-His tagged protein (11142)  in a linear range of 62.5-4000 ng/ml.

Figure 4. HEK293 cell line transfected with irrelevant protein  (B) and human B7-2  (A) were surface stained with Human CD28, mFc-His tagged protein (11142) 1µg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
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