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Human CD3D Protein, His x Tag and Human CD3E Protein, hFc Tag

Recombinant Human CD3D Protein with C-terminal 6XHis tag and C-terminal human Fc tag


Cat.#:  11520

   Size:   100 µg

In Stock

          Product Description          
Cat.#:     11520
Product Name:   Human CD3D Protein; His Tag And Human CD3E Protein; HFc Tag
Size :    10 µg; 50 µg and 100 µg
Synonyms:   CD3-DELTA;IMD19;T3D and IMD18;T3E;TCRE
Target:    CD3E
UNIPROT ID:    P04234;P07766
Description:    Recombinant Human CD3D Protein With C-Terminal 6×His Tag And Human CD3E Protein With C-Terminal Human Fc Tag
Background:     T-cell surface glycoprotein CD3 delta and CD3 epsilon chain, also known as CD3D and CD3E or CD3DandCD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 10.4 and 37.9 kDa after removal of the signal peptide. The apparent molecular mass of CD3D-His and CD3E-hFc is approximately 35-55 kDa due to glycosylation.
Molecular Characterization:    CD3D(Phe22-Ala105) 6×His tag and CD3E(Asp23-Asp126) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HC, 150 mM NaC, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD3D&CD3E Heterodimer Protein, His Tag & hFc Tag on SDS-PAGE under reducing condition.