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Human CD3E Protein, hFc Tag

Recombinant human CD3E protein with C-terminal human Fc tag
5/5

$415.00

Cat.#:  11609

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11609
Product Name:   Human CD3E Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD3e;T3E
Target:    CD3E
UNIPROT ID:    P07766
Description:    Recombinant human CD3E protein with C-terminal human Fc tag
Background:     The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 37.9 kDa after removal of the signal peptide. The apparent molecular mass of CD3E-hFc is approximately 35-55 kDa due to glycosylation.
Molecular Characterization:    CD3E(Asp23-Asp126) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD3E Protein, hFc Tag on SDS-PAGE under reducing condition.
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