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Human CD73 Protein, hFc Tag

Recombinant Human CD73 Protein with C-terminal human Fc tag


Cat.#:  11610

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11610
Product Name:   Human CD73 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CALJA;CD73;E5NT;eN;eNT;NT;NT5;NTE
Target:    CD73
UNIPROT ID:    P21589
Description:    Recombinant Human CD73 Protein with C-terminal human Fc tag
Background:     The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 84.1 kDa after removal of the signal peptide.The apparent molecular mass of CD73-hFc is approximately 55-100 kDa due to glycosylation.
Molecular Characterization:    CD73(Trp27-Ser549) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD73 Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD73 Protein, hFc Tag (11610) can bind Anti-CD73 Neutralizing antibody 28124 in a linear range of 3.66-937.50 ng/mL.