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Human FLT1 Protein, His Tag

Recombinant human FLT1 protein with C-terminal 6XHis tag


Cat.#:  11516

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11516
Product Name:   Human FLT1 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   VEGFR-1;Tyrosine-protein kinase FRT;FLT-1;FLT;Vascular permeability factor receptor
Target:    FLT1
UNIPROT ID:    P17948
Description:    Recombinant human FLT1 protein with C-terminal 6xHis tag
Background:     This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.[provided by RefSeq, May 2009]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 83.0 kDa after removal of the signal peptide. The apparent molecular mass of FLT1-His is approximately 100-130 kDa due to glycosylation.
Molecular Characterization:    FLT1(Ser27-Asn756) 6×His tag
Purity:    The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human  FLT1 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human FLT1 Protein, His Tag (11516) can bind Anti-FLT1 Neutralizing antibody 28126 in a linear range of 0.92-234.38 ng/mL.