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Human GITR Protein, hFc-His tag

Recombinant human GITR protein with C-terminal human Fc and 6XHis tag


Cat.#:  11146

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11146
Product Name:   Human GITR Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   AITR;GITR;TNFRSF18;CD357
Target:    GITR
Description:    Recombinant human GITR protein with C-terminal human Fc and 6xHis tag
Background:     This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25( )CD4( ) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 51-52 kDa after removal of the signal peptide.
Molecular Characterization:    GITR(Gln26-Pro162) hFc(Glu99-Ala330) 6×His
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human GITR Protein, hFc-His Tag on SDS-PAGE under reducing condition.

Figure 2.  Flow cytometry analysis with 1 µg/ml Human GITR Protein, hFc-His tag (11146) on Expi293 cells transfected with human GITRL (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).