| Cat.#: 11146 |
| Product Name: Human GITR Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: AITR;GITR;TNFRSF18;CD357 |
| Target: GITR |
| UNIPROT ID: Q9Y5U5 |
| Description: Recombinant human GITR protein with C-terminal human Fc and 6xHis tag |
| Background: This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25( )CD4( ) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 51-52 kDa after removal of the signal peptide. |
| Molecular Characterization: GITR(Gln26-Pro162) hFc(Glu99-Ala330) 6×His |
| Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human GITR Protein, hFc-His Tag on SDS-PAGE under reducing condition. | 
Figure 2. Flow cytometry analysis with 1 µg/ml Human GITR Protein, hFc-His tag (11146) on Expi293 cells transfected with human GITRL (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram). |
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