Cat.#: 11253 |
Product Name: Human HVEM Protein |
Size : 10 µg, 50 µg and 100 µg |
Synonyms: ATAR;CD270;HVEA;HVEM;LIGHTR;TR2 |
Target: HVEM |
UNIPROT ID: Q92956 |
Description: Recombinant Human HVEM Protein with C-terminal 6xHis tag |
Background: This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants. |
Species/Host: HEK293 |
Molecular Weight: The protein has a predicted molecular mass of 18.2 kDa after removal of the signal peptide.The apparent molecular mass of HVEM-His is approximately 25-55 kDa due to glycosylation. |
Molecular Characterization: HVEM(Leu39-Val202) 6×His tag |
Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
 Figure 1. Human HVEM Protein, His Tag on SDS-PAGE under reducing condition. |  Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human HVEM, His tagged protein (11253) can bind Human BTLA,mFc-His tagged protein 11174 in a linear range of 0.64-80 ng/ml. |  Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human HVEM, His tagged protein (11253) can bind Human CD160,hFc tagged protein 11179 in a linear range of 1.28-160 ng/ml. |  Figure 4. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human HVEM, His tagged protein (11253) can bind Human LIGHT, hFc tagged protein 11175 in a linear range of 0.32-40 ng/ml. |  Figure 6. Flow cytometry analysis with 15 µg/ml Human HVEM Protein, His tag (11253) on Expi293 cells transfected with human LIGHT (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram). |  Figure 6. Flow cytometry analysis with 15 µg/ml Human HVEM Protein, His tag (11253) on Expi293 cells transfected with human LIGHT (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram). |
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