| Cat.#: 11735 |
| Product Name: Human IL1A Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: IL-1 alpha;IL-1A;IL1;IL1-ALPHA;IL1F1 |
| Target: IL1A |
| UNIPROT ID: P01583 |
| Description: Recombinant Human IL1A Protein with C-terminal human Fc tag |
| Background: The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008] |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 44.2 kDa after removal of the signal peptide.The apparent molecular mass of IL1A-hFc is approximately 35-55 kDa due to glycosylation. |
| Molecular Characterization: IL1A(Ser113-Ala271) hFc(Glu99-Ala330) |
| Purity: The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human IL1A Protein, hFc Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human IL1A Protein, hFc Tag ( 11735) can bind Anti-IL1A Neutralizing antibody 28097 in a linear range of 3.05-740.74 ng/mL. |
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