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Human IL2RA Protein, His Tag

Recombinant human IL2RA Protein with C-terminal 6XHis tag


Cat.#:  11217

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11217
Product Name:   Human IL2RA Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   IL2RA;D25;55;L2-RA;L-2-RA
Target:    IL2RA
UNIPROT ID:    P01589
Description:    Recombinant human IL2RA Protein with C-terminal 6xHis tag
Background:     The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 22.6 kDa after removal of the signal peptide.The apparent molecular mass of IL2RA-His is approximately 35-55 kDa due to glycosylation.
Molecular Characterization:    IL2RA(Glu22-Cys213) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human IL2RA Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human IL2RA, His tagged protein (11217) can bind Anti-IL2RA Neutralizing antibody 28052 in a linear range of 0.64-400 ng/ml.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human IL2RA Protein, His Tag (11217) can bind Human IL2 Protein, hFc Tag 11256 in a linear range of 0.64-400 ng/mL.