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Human IL4RA Protein, hFc Tag

Recombinant human IL4RA Protein with C-terminal Human Fc tag


Cat.#:  11593

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11593
Product Name:   Human IL4RA Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD124;IL-4RA;IL4RA
Target:    IL4RA
UNIPROT ID:    P24394
Description:    Recombinant human IL4RA Protein with C-terminal Human Fc tag
Background:     This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 49.9 kDa after removal of the signal peptide.The apparent molecular mass of IL4RA-hFc is approximately 55-70 kDa due to glycosylation.
Molecular Characterization:    IL4RA(Met26-His232) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human IL4RA Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human IL4RA Protein, hFc Tag(11593) can bind Anti-IL4RA antibody(DMC284), IgG1 Chimeric mAb in a linear range of 16.0-400 ng/mL.