| Cat.#: 11318 |
| Product Name: Human MICB Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: MIC-B;PERB11.2 |
| Target: MICB |
| UNIPROT ID: Q29980 |
| Description: Recombinant Human MICB Protein with C-terminal 6xHis tag |
| Background: This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules, however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 33.5 kDa after removal of the signal peptide.The apparent molecular mass of MICB-His is approximately 35-55 kDa due to glycosylation. |
| Molecular Characterization: MICB(Ala23-Asp309) 6×His tag |
| Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human MICB Protein, His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human MICB protein, His Tag (11318) can bind Anti-MICB Rabbit mAb in a linear range of 0.128-16 ng/mL. | 
Figure 3. Flow cytometry analysis with 15 µg/ml Human MICB Protein, His Tag (11318) on Expi293 cells transfected with Human NKG2D protein and Human DAP10 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram). | |
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