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Human MICB Protein, His Tag

Recombinant Human MICB Protein with C-terminal 6XHis tag
5/5

$525.00

Cat.#:  11318

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11318
Product Name:   Human MICB Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   MIC-B;PERB11.2
Target:    MICB
UNIPROT ID:    Q29980
Description:    Recombinant Human MICB Protein with C-terminal 6xHis tag
Background:     This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules, however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 33.5 kDa after removal of the signal peptide.The apparent molecular mass of MICB-His is approximately 35-55 kDa due to glycosylation.
Molecular Characterization:    MICB(Ala23-Asp309) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human MICB Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human MICB protein, His Tag (11318) can bind Anti-MICB Rabbit mAb in a linear range of 0.128-16 ng/mL.

Figure 3. Flow cytometry analysis with 15 µg/ml Human MICB Protein, His Tag (11318) on Expi293 cells transfected with Human NKG2D protein and Human DAP10 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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