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Human OX40L Protein, mFc-His tag

Recombinant human OX40L protein with N-terminal mouse Fc and C-6XHis tag
5/5

$555.00

Cat.#:  11149

   Size:   100 μg

In Stock

            Product Description          
Cat.#:     11149
Product Name:   Human OX40L Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   OX40L;TNFSF4;CD252;Glycoprotein Gp34;TXGP1;CD134 ligand;CD134L
Target:    OX40 Ligand
UNIPROT ID:    P23510
Description:    Recombinant human OX40L protein with N-terminal mouse Fc and C-6xHis tag
Background:     This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 44.0 kDa after removal of the signal peptide.
Molecular Characterization:    mFc(Pro99-Lys330) OX40 Ligand(Gln51-Leu183) 6×His
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human OX40 Ligand Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. HEK293 cell line transfected with irrelevant protein  (B) and human OX40  (A) were surface stained with Human OX40 Ligand, mFc-His tagged protein (11149) 10 µg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Figure 3. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human OX40-L Protein, mFc-His Tag (11149) can bind Anti-OX40-L Rabbit mAb in a linear range of 3.2-80 ng/mL.
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