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Human SIRPa Protein, mFc Tag

Recombinant Human SIRPa with C-terminal mouse Fc tag


Cat.#:  11331

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11331
Product Name:   Human SIRPa Protein
Size :    10 µg, 50 µg and 100 µg
Target:    SIRPa
UNIPROT ID:    P78324
Description:    Recombinant Human SIRPa with C-terminal mouse Fc tag
Background:     Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 63.8 kDa after removal of the signal peptide.The apparent molecular mass of SIRPa-mFc is approximately 70-100 kDa due to glycosylation.
Molecular Characterization:    SIRPa(Glu31-Tyr373) mFc(Pro99-Lys330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human SIRPα Protein, mFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human SIRPα Protein, mFc Tag  (11331) can bind anti-SIRPα antibody, Rabbit mAb clone: DM8 in a linear range of 3.2-80 ng/mL.