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Human TNFa Protein, His Tag

Recombinant human TNFa protein with C-terminal 6XHis tag


Cat.#:  11199

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11199
Product Name:   Human TNFa Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   DIF;NF-alpha;NFA;NFSF2;achexin;achectin;NFa
Target:    TNFA
UNIPROT ID:    P01375
Description:    Recombinant human TNFa protein with C-terminal 6xHis tag
Background:     This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide.
Molecular Characterization:    TNFa(Val77-Leu233) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human TNFα Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human TNFa , His tagged protein (11199) can bind Anti-TNFa Neutralizing antibody 28064 in a linear range of 0.64-16 ng/ml.