| Cat.#: 28387 |
| Product Name: Anti-Mesothelin(DM73) Rabbit Monoclonal Antibody |
| Synonyms: MSLN; Mesothelin; MPF |
| Description: Anti-mesothelin antibody(DM73) Rabbit Monoclonal Antibody |
| Background: This gene encodes a preproprotein that is proteolytically processed to generate two protein products; megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas; ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants; at least one of which encodes an isoform that is proteolytically processed. |
| Applications: ELISA; Flow Cyt |
| Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100 |
| Host Species: Rabbit |
| Isotype: Rabbit IgG |
| Purification: Purified from cell culture supernatant by affinity chromatography |
| Species Reactivity: Human Mesothelin |
| Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). |
Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human MSLN protein, mFc-His tagged protein 11159 can bind Rabbit anti-MSLN monoclonal antibody ( clone: DM73) in a linear range of 1-100 ng/ml. | 
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human mesothelin (B) were surface stained with Rabbit anti-MSLN monoclonal antibody 1µg/ml ( clone: DM73) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody. | 
Figure 3. Flow cytometry data of serially titrated Rabbit anti-MSLN monoclonal antibody ( clone: DM73) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. | 
Figure 4. Affinity ranking of different Rabbit anti-MSLN mAb clones by titration of different concentration onto Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. |
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