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Mouse EGFR Protein, His Tag

Recombinant mouse EGFR protein with C-terminal 6XHis tag
5/5

$569.00

Cat.#:  12186

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     12186
Product Name:   Mouse EGFR Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   Epidermal growth factor receptor;Egfr
Target:    EGFR
UNIPROT ID:    Q01279
Description:    Recombinant mouse EGFR protein with C-terminal 6xHis tag
Background:     Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:8404850). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (By similarity). Plays a role in enhancing learning and memory performance (PubMed:20639532).[UniProtKB/Swiss-Prot Function]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 70.1kDa after removal of the signal peptide. The apparent molecular mass of mEGFR-His is approximately 100-130 kDa due to glycosylation.
Molecular Characterization:    Mouse EGFR(Leu25-Ser647) 6×His tag
Purity:    The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Mouse EGFR Protein, His Tag on SDS-PAGE under reducing condition.
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