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Human MRGPRX2 Full Length Protein

Human MRGPRX2 Full Length Protein-Synthetic Nanodisc
5/5

$4,400.00

Cat.#:  11086

   Size:   50 μg

In Stock

          Product Description          
Cat.#:     11086
Product Name:   Human MRGPRX2 Full Length Protein
Size :    10 µg; 50 µg and 100 µg
Synonyms:   MGRG3; MRGX2
Target:    MRGPRX2
UNIPROT ID:    Q96LB1
Description:    Human MRGPRX2 Full Length Protein-Synthetic Nanodisc
Background:     Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core. Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction. Acts as a receptor for a number of other ligands, including peptides and alkaloids, such as cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and, at lower extent, PAMP-20, antibacterial protein LL-37, PMX-53 peptide, beta-defensins, and complanadine A.
Species/Host:    HEK293
Molecular Weight:    The human full length MRGPRX2 protein has a MW of 37.1 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Protein Families:    Druggable Genome, GPCR, Transmembrane
Protein Pathways:    N.A.

Figure 1. Elisa plates were pre-coated with Flag Tag MRGPRX2-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with MRGPRX2-Nanodisc is 1.523ng/ml.

Figure 2. Human MRGPRX2-Nanodisc, Flag Tag on SDS-PAGE
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