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PD-1 mAb (Pembrolizumab)

Anti-PD-1? Monoclonal Antibody (PembrolizuMonoclonal Antibody)?


Cat.#:  28015

   Size:   100 μg

In Stock

            Product Description          
Cat.#:   28015
Product Name:   Anti-PD-1 (Pembrolizumab) Monoclonal Antibody
Synonyms:   PDCD1;PD1;CD279;SLEB2
Description:    Anti-PD-1 (pembrolizumab ) Monoclonal Antibody
Background:    Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Humanized
Isotype:    IgG4
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human PD-1
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1 . ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human PD1, mFc-His tagged protein 11153 can bind Anti-PD-1 Neutralizing antibody in a linear range of 0.24-6.49 ng/ml.

Figure 2. HEK293 cell line transfected with irrelevant protein  (B) and human PD1  (A) were surface stained with anti-PD1 neutralizing antibody 1µg/ml (pembrolizumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.