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Rab35-GTP

Anti-Rab35-GTP Monoclonal Antibody
5/5

$495.00

Cat.#:  26922

   Size:   30 μl

In Stock

          Product Description          
Anti-Rab35GTP Monoclonal Antibody
Cat. #:   26922
Size:  30 µL
Gene Symbol:  RAB35
Description:   Anti-Rab35-GTP Monoclonal Antibody
Background:   The Rab family of GTPases regulates eukaryotic vesicular membrane traffic. Rab35 is with both plasma membrane and endosomal localization, and has been implicated in diverse processes that include T-cell receptor recycling, oocyte yolk protein recycling and cytokinesis. Rab35 regulates neurite outgrowth in neuronal-like cells, and can induce protrusions.
Immunogen:   Recombinant full length protein of active Rab35
Applications:   IP, IHC and IF (Not applicable for WB since SDS denatures Rab35 GTPase)
Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Rab35-GTP monoclonal antibody recognizes active Rab35 proteins of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:   Rab35 Activation Assay. Purified full-length Rab35 proteins were immunoprecipitated after treated with GDP (lane 1) orGTPγS (lane 2). Immunoprecipitation was done with the anti-Rab35-GTP monoclonal antibody (Cat. No. 26922). Immunoblot was with an anti-Rab35 polyclonal antibody (Cat. No. 21078).
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