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Rap1B Protein

Rap1B Protein
5/5

$347.00

Cat.#:  10120

   Size:   100 μl

In Stock

            Product Description          

Rap1B Protein

Cat. #:  10120
Product Name:  Rap1B Protein
Synonyms:  Member of RAS oncogene family, K-REV, RAL1B
Source:   Human, recombinant full length, His6-tag
Expression Host Species:   E. coli
Molecular Weight:   21 kDa
Purity:   >95% by SDS-PAGE
Introduction:   Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway.
Amino Acid Sequence   (1-184)
MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAM
RDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNL
ARQWNNCAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSSCQLL
Properties
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):  White or clear
Concentration:  1 mg/mL
Storage:   -80°C
Preparation Instructions:  
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rap1B was determined by SDS- PAGE and Coomassie Brilliant Blue Staining


References:  
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