NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
Cat. #: 26036 |
Gene Symbol: RAS(G12D) |
Description: Anti-Ras(G12D) Mouse Monoclonal Antibody |
Background: Ras (G12D) is one type of Ras missense mutations at the codon 12. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G12D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers. |
Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of G12D, human origin |
Applications: ELISA, WB, IF, IHC |
Recommended Dilutions: ELISA: 1:100-1:3000 WB: 1:100-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG1, IgG2a, IgG2b |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Ras(G12D) antibody recognizes Ras(G12D) proteins of vertebrates |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of recombinant Ras WT and G12D proteins. Equal amounts of purified Ras WT (lane 1, Cat # 10172) and G12D (lane 2, Cat # 10154) proteins were loaded and blotted with anti-Ras G12D mouse monoclonal antibody (Cat # 26036) (Top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti-Ras antibody (Cat # 21021). Immunofluorescence: Immunofluorescence of cells expressing RAS proteins with anti-RAS (G12D) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(WT) plasmid (left column) or pCDNA3-GFP Ras(G12D) plasmid (right column), then fixed and stained with anti-Ras(G12D) monoclonal antibody (Cat. # 26036). |