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RET(C634Y)

Anti-RET(C634Y) Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26146

   Size:   100 μl

In Stock

          Product Description          
RET(C634Y)
Cat. #:  26146
Gene Symbol:  RET, PTC, CDHF12, CAHF16, HSCR1, MEN2A, MEN2B
Description:   Anti-RET(C634Y) Mouse Monoclonal Antibody
Background:   RET protein is encoded by the RET proto- oncogene. It is a receptor tyrosine kinase. RET loss of function mutations are associated with the development of Hirschsprung’s disease, while gain of function mutations are associated with development of various types of human cancer, including medullary thyroid carcinoma, multiple endocrine neoplasia type 2A and 2B, pheochromocytoma and parathyroid hyperplasia.
Immunogen:   A synthetic peptide from the internal region of RET which includes the mutation of C634Y, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:    ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100
Concentration:   0.5 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes C634Y mutant, but not wild type RET of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant RET(C634Y) and wild type proteins. Purified His-tagged RET(C634Y) protein (lane1) and corresponding wild type protein (lane2) were blotted with Anti-RET(C634Y) monoclonal antibody (Cat. #26146).
Immunoprecipitation/Western blot:   Immunofluorescence of cells expressing RET proteins with Anti-RET(C634Y) antibody. HEK293T cells were transfected with pCDNA3-GFP-RET (WT) plasmid (left column) or pCDNA3-GFP-RET(C634Y) plasmid (right column), then fixed and stained with Anti-RET(C634Y) monoclonal antibody (Cat. #26146).
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