NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
Anti-RhebGTP Monoclonal Antibody |
Cat. #: 26910 |
Size: 30 µL |
Gene Symbol: RHEB |
Description: Anti-Rheb-GTP Monoclonal Antibody |
Background: Small GTPases are a super-family of cellular signaling regulators. Rheb is a member of the Ras-superfamily GTPases. Rheb has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related (ATR). |
Immunogen: Recombinant full-length protein of active Rheb. |
Applications: IP, IHC, IF(Not applicable for WB since SDS denatures Rheb GTPase) |
Recommended Dilutions: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgM |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Anti-Rheb-GTP monoclonal antibody recognizes active Rheb of vertebrates. |
Storage Conditions: Store at -20°C. Avoid freeze/thaw cycles |
Immunoprecipitation/Western blot: Rheb activation assay. Purified Rheb proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rheb-GTP monoclonal antibody (Cat. No. 26910). Immunoblot was with an anti-Rheb rabbit polyclonal antibody (Cat. No. 21098). |