NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$239.00
Cat. #: 26453 |
Gene Symbol: SMAD4, DPC4, JIP, MADH4, MYHRS |
Description: Anti-SMAD4 Mouse Monoclonal Antibody |
Background: SMAD4 family member 4, also known as SMAD4, is a protein that in human is encoded by the SMAD4 gene. It is often found mutated in many cancers. It acts as a tumor suppressor that functions in the regulation of the TGF-β signal transduction pathway, which negatively regulates growth of epithelial cells and the extracellular matrix (ECM). SMAD4 alterations have been found in multiploid colorectal cancer and pancreatic carcinoma. It is found inactivated in at least 50% of pancreatic cancers. It is also found mutated in the autosomal dominant disease juvenile polyposis syndrome (JPS). |
Immunogen: Purified SMAD4 protein, human origin. . |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes SMAD4 of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of recombinant SMAD4 protein. Purified His-tagged SMAD4 protein (1-552aa, 65kd) and was blotted with anti-SMAD4 monoclonal antibody (Cat. #26453). Immunofluorescence: Immunofluorescence of cells expressing SMAD4 proteins with anti SMAD4 antibody. HEK293T cells were transfected with pCDNA3-GFP-SMAD4 (WT) plasmid, then fixed and stained with anti SMAD4 monoclonal antibody (Cat. #26453). |