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Human TRPA1 Full Length Protein

Human TRPA1 Full Length Protein-Synthetic Nanodisc
5/5

$4,400.00

Cat.#:  11016

   Size:   50 μg

In Stock

            Product Description          
Cat.#:     11016
Product Name:   Human TRPA1 Full Length Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   ANKTM1; FEPS; FEPS1
Target:    TRPA1
UNIPROT ID:    O75762
Description:    Human TRPA1 full length protein-synthetic nanodisc
Background:     The structure of the protein is highly related to both the protein ankyrin and transmembrane proteins. This protein is activated by a large variety of structurally unrelated electrophilic and non-electrophilic chemical compounds. Electrophilic ligands activate TRPA1 by interacting with critical N-terminal Cys residues in a covalent manner, whereas mechanisms of non-electrophilic ligands are not well determined. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.
Species/Host:    HEK293
Molecular Weight:    The human full length TRPA1 protein has a MW of 127.5 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag TRPA1-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with TRPA1-Nanodisc is 7.433ng/ml.

Figure2. Human TRPA1-Nanodisc, Flag Tag on SDS-PAGE
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