NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$495.00
CTNNB1(S45P)
Cat. #: 26307 |
Gene Symbol: Beta catenin, CTNNB, Catenin beta-1 |
Description: Anti-CTNNB1(S45P) Mouse Monoclonal Antibody |
Background: CTNNB1 protein is a dual function protein. It is a subunit of a complex of proteins that from adherent Anti-CTNNB1(S45P) Mouse Monoclonal Antibody junctions, which are important for the establishment and maintenance of epithelial cell layers by regulating cell growth and adhesion between adjacent cells. CTNNB1 protein also pulls double duty as an intracellular signal transducer in the Wnt signaling pathway. Mutations of CTNNB1 have been implicated in the pathogenesis of several cancers. |
Immunogen: A synthetic peptide from the internal region of CTNNB1 which includes the mutation of S45P, human origin. |
Applications: ELISA, IF, IHC |
Recommended Dilutions: ELISA: 1:1000-1:2000 IF: 1:50-1:100 IHC: 1:50-1:100 |
Concentration: 0.5 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes S45P mutant, but not wild type CTNNB1 of vertebrates. |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Immunofluorescence of cells expressing CTNNB1 proteins with anti CTNNB1(S45P) antibody. HEK293T cells were transfected with pCDNA3-GFP CTNNB1(S45P) plasmid, pCDNA3-GFP CTNNB1(WT) plasmid pCDNA3-GFP CTNNB1(S45F) plasmid or pCDNA3-GFP CTNNB1(S45Y) plasmid, then fixed and stained with anti CTNNB1(S45P) monoclonal antibody (Cat. #26307). |