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Arf6-GTP

Anti-Arf6-GTP Monoclonal Antibody
5/5

$495.00

Cat.#:  26918

   Size:   30 μl

In Stock

          Product Description          
Anti-Arf6GTP Monoclonal Antibody
Cat. #:  26918
Size:  30 µL
Gene Symbol:  ARF6
Description:   Anti-Arf6-GTP Monoclonal Antibody
Background:   ARF6 (ADP-ribosylation factor 6) is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and functions as a regulatory molecule of phagocytosis.
Immunogen:   Recombinant full length protein of active Arf6.
Applications:   IP, IHC and IF (Not applicable for WB since SDS denatures Arf6 GTPase)
Published Applications:  IP, IHC and IF – Click for Details
Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:    1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:    Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-Arf6-GTP monoclonal antibody recognizes active Arf6 from vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:  
Arf6 activation assay. Purified full-length Arf6 proteins were immunoprecipitated after treated with GDP (lane left) or GTPγS (lane right). Immunoprecipitation was done with the anti-Arf6-GTP monoclonal antibody (Cat. No. 26918). Immunoblot was with an anti-Arf6 polyclonal antibody (Cat. No. 21032).
          Publications          
01. Tumor- and Nerve-Derived Axon Guidance Molecule Promotes Pancreatic Ductal Adenocarcinoma Progression and Metastasis through Macrophage Reprogramming
bioRxiv. 2023  PMID: 37961340
02. Trimming nano delivery at the cell side by tuning ARF6 GTPases to the GTP-bound form
Research Square. 2022  PMID:
03. PSD3 downregulation confers protection against fatty liver disease
Nat Metab. 2022  PMID: 35102341
04. Small GTPase ARF6 Is a Coincidence-Detection Code for RPH3A Polarization in Neutrophil Polarization
J Immunol. 2020  PMID:  31924649
05. A novel role for the late-onset Alzheimer’s disease (LOAD)-associated protein Bin1 in regulating postsynaptic trafficking and glutamatergic signaling
Mol Psychiatry. 2020  PMID:  30967682
06. Small GTPase ARF6 Is a Coincidence-Detection Code for RPH3A Polarization in Neutrophil Polarization
J Immunol. 2020  PMID: 31924649
07. Acetylation of ACAP4 regulates CCL18-elicited breast cancer cell migration and invasion
J Mol Cell Biol. 2018  PMID: 30395269
08. ELMOD1 Stimulates ARF6-GTP Hydrolysis to Stabilize Apical Structures in Developing Vestibular Hair Cells
J Neurosci. 2018  PMID: 29222402
09. The cytohesin guanosine exchange factors (GEFs) are required to promote HGF-mediated renal recovery after acute kidney injury (AKI) in mice
Physiol Rep. 2015  PMID: 26116550
10. Arf6 Guanine Nucleotide Exchange Factor Cytohesin-2 Binds to CCDC120 and Is Transported Along Neurites to Mediate Neurite Growth
J Biol Chem. 2014  PMID: 25326380