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EGFR (DM130) Rabbit mAb

Anti-EGFR (DM130) Rabbit Monoclonal Antibody
5/5

$445.00

Cat.#:  28475

   Size:   100 μg

In Stock

          Product Description          
Cat.#:   28475
Product Name:   Anti-EGFR(DM130) Rabbit Monoclonal Antibody
Synonyms:   EGFR;ERBB;ERBB1;HER1;PIG61;mENA
Description:    Anti-EGFR antibody(DM130) Rabbit Monoclonal Antibody
Background:    The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Rabbit
Isotype:    Rabbit IgG
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human EGFR
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human EGFR protein, His tagged protein 11218 can bind Rabbit anti-EGFR monoclonal antibody (clone: DM130) in a linear range of 0.06-12 ng/ml.

Figure 2. EGFR protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EGFR  (DM130) on Expi293 cells transfected with human EGFR(Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram),and  Isotype antibody on Expi293 transfected with irrelevant protein(Orange histogram)
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