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Anti-FBXW7(R479L) Mouse Monoclonal Antibody


Cat.#:  26347

   Size:   100 μl

In Stock

            Product Description          
Cat. #:  26347
Gene Symbol:  FBW6; FBW7; FBXW7; FBX30; CDC4; SEL10
Description:   Anti-FBXW7(R479L) Mouse Monoclonal Antibody
Background:   F-box/WD repeat-containing protein 7 is a protein that in humans is encoded by the FBXW7 gene. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Mutations in this gene are detected in ovarian, breast and colorectal cancer cell lines, implicating the gene’s potential role in the pathogenesis of human cancers.
Immunogen:   A synthetic peptide from the internal region of FBXW7 which includes the mutation of R479L, human origin.
Applications:   ELISA, WB
Recommended Dilutions:    ELISA: 1:1000-1:5000 WB: 1:500-1:1000
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes R479L mutant, but not wild type FBXW7 of vertebrates.
Storage Conditions:   Store at -20°C. Avoid repeated freezing and thawing
Western blot:   Western blot analysis of recombinant FBXW7(R479L) and wild type proteins. Purified His-tagged FBXW7(R479L) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-FBXW7(R479L) monoclonal antibody (Cat. #26347). Immunofluorescence:   Immunofluorescence of cells expressing FBXW7 proteins with Anti-FBXW7(R479L) antibody. HEK293T cells were transfected with pCDNA3-GFP-FBXW7 (WT) plasmid (left column) or pCDNA3-GFP-FBXW7(R479L) plasmid (right column), then fixed and stained with Anti-FBXW7(R479L) monoclonal antibody (Cat. #26347).
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