$495.00
| Cat. #: 26347 |
| Gene Symbol: FBW6; FBW7; FBXW7; FBX30; CDC4; SEL10 |
| Description: Anti-FBXW7(R479L) Mouse Monoclonal Antibody |
| Background: F-box/WD repeat-containing protein 7 is a protein that in humans is encoded by the FBXW7 gene. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Mutations in this gene are detected in ovarian, breast and colorectal cancer cell lines, implicating the gene’s potential role in the pathogenesis of human cancers. |
| Immunogen: A synthetic peptide from the internal region of FBXW7 which includes the mutation of R479L, human origin. |
| Applications: ELISA, WB |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 |
| Concentration: 1 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes R479L mutant, but not wild type FBXW7 of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot:
Western blot analysis of recombinant FBXW7(R479L) and wild type proteins.
Purified His-tagged FBXW7(R479L) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-FBXW7(R479L) monoclonal antibody (Cat. #26347).
Immunofluorescence:
Immunofluorescence of cells expressing FBXW7 proteins with Anti-FBXW7(R479L) antibody.
HEK293T cells were transfected with pCDNA3-GFP-FBXW7 (WT) plasmid (left column) or pCDNA3-GFP-FBXW7(R479L) plasmid (right column), then fixed and stained with Anti-FBXW7(R479L) monoclonal antibody (Cat. #26347). |
