$239.00
Gαi Monoclonal Antibody
Cat. #: 26003 |
Gene Symbol: Gnai |
Description: Anti-Gαi Mouse Monoclonal Antibody |
Background: Heterotrimeric G proteins are critical cellular signal transducers. Gai represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gai proteins are being explored. |
Immunogen: Recombinant full length protein of Gαi1 |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA 1:1000-1:5000,WB 1:1000-1:2000,IHC 1:100-1:200 |
Concentration: 1 mg/ml |
Host Species: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Preservative: No |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Gαi antibody recognizes Gαi1, Gαi2, and Gαi3 of vertebrates |
Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
Western blot: Western blot analysis of Gi proteins. Gαi1 plasmid DNA was transiently transfected into HEK-293 cells. Whole-cell lysate was used for western blots. The monoclonal anti-Gαi antibody was pre-incubated without (lane 1) or with (lane 2) the antigen protein (Gαi1) to show the specificity. |