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o-GTP

Anti-Gαo-GTP Mouse Monoclonal Antibody
5/5

$495.00

Cat.#:  26907

   Size:   30 μl

In Stock

          Product Description          
Anti  GαoGTP Mouse Monoclonal Antibody
Cat. #:   26907
Size:  30 µL
Gene Symbol:  Gnao
Description:   Anti  Gαo-GTP Mouse Monoclonal Antibody
Background:   Heterotrimeric G proteins are critical cellular signal transducers. Gαo represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gαo proteins are being explored.
Immunogen:   Recombinant full length protein of active Gαo
Applications:   IP, IHC and IF (Not applicable for WB since SDS denatures Gαo GTPase)
Recommended Dilutions:    IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG1
Purity:   Purified from ascites
Preservative:  No
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-active Gαo antibody recognizes active Gαo of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing
Immunoprecipitation/Western blot:   o Activation Assay. Purified Gαo proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Gαo monoclonal antibody (Cat. No. 26907). Immunoblot was with an anti-Gαopolyclonal antibody (Cat. No. 21015).
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