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Human B7-1 Protein, mFc-His Tag

Recombinant Human B7-1 Protein with C-terminal Mouse Fc and 6XHis tag
5/5

$555.00

Cat.#:  11173

   Size:   100 μg

In Stock

            Product Description          
Cat.#:     11173
Product Name:   Human B7-1 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD80;7;7-1;7.1;B1;D28LG;D28LG1;AB7
Target:    B7-1
UNIPROT ID:    P33681
Description:    Recombinant Human B7-1 Protein with C-terminal Mouse Fc and 6xHis tag
Background:     The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of B7-1-mFc-His is approximately 70-130 kDa due to glycosylation.
Molecular Characterization:    B7-1(Val35-Asn242) mFc(Pro99-Lys330) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human B7-1 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human B7-1, mFc-His tagged protein  (11173) can bind  anti-B7-1 monoclonal antibody,Rabbit mAb clone: DM111 in a linear range of 0.13-80 ng/ml.

Figure 3.  Flow cytometry analysis with 15 µg/ml Human B7-1 Protein, mFc-His tag (11173) on Expi293 cells transfected with human CD28 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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