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Human B7-H4 Protein, hFc Tag

Recombinant Human B7-H4 protein with C-terminal human Fc


Cat.#:  11177

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11177
Product Name:   Human B7-H4 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   B7-H4;B7h.5;B7H4;B7S1;B7X;PRO1291;VCTN1
Target:    B7-H4
Description:    Recombinant Human B7-H4 protein with C-terminal human Fc
Background:     This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 51.4 kDa after removal of the signal peptide.The apparent molecular mass of B7-H4-hFc is approximately 100-130 kDa due to glycosylation.
Molecular Characterization:    B7-H4 (Phe29-Ala258) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human B7-H4 Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human B7-H4, hFc-His tagged protein  (11177) can bind Anti-B7-H4 Neutralizing antibody 28081 in a linear range of 3.2-80 ng/ml.