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Human CB1 Protein, hFc Tag

Recombinant Human CB1 protein with C-terminal human Fc


Cat.#:  11311

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11311
Product Name:   Human CB1 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CANN6;CB-R;CB1;CB1A;CB1K5;CB1R;CNR
Target:    CB1
UNIPROT ID:    P21554
Description:    Recombinant Human CB1 protein with C-terminal human Fc
Background:     This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 44.2 kDa after removal of the signal peptide.The apparent molecular mass of CB1-hFc is approximately 70-100 kDa due to glycosylation.
Molecular Characterization:    CB1(Met1-Gln116) (Asp176-Asn187) (Asn256-Glu273) (Asp366-Thr377) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CB1 Protein, hFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CB1 Protein, hFc Tag (11311) can bind Anti-CB1 antibody(DM144), Rabbit mAb in a linear range of 3.20-16 ng/mL.