| Cat.#: 11203 |
| Product Name: Human CD114 Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: CSF3R;D114;CSFR |
| Target: CD114 |
| UNIPROT ID: Q99062 |
| Description: Recombinant human CD114 protein with C-terminal 6xHis tag |
| Background: The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Alternatively spliced transcript variants have been described. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 67.5 kDa after removal of the signal peptide.The apparent molecular mass of CD114-His is approximately 100-110 kDa due to glycosylation. |
| Molecular Characterization: CD114(Glu25-Pro622) 6×His tag |
| Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human CD114 Protein, His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human CD114 protein, His Tag (11203) can bind Anti-CD114 Rabbit mAb in a linear range of 0.128-16 ng/mL. |
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