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Human CD138 Protein, hFc-His Tag

Recombinant human CD138 protein with C-terminal human Fc and 6XHis tag


Cat.#:  11134

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11134
Product Name:   Human CD138 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   SDC1;Syndecan-1;CD138;SYND1;SDC
Target:    CD138
UNIPROT ID:    P18827
Description:    Recombinant human CD138 protein with C-terminal human Fc and 6xHis tag
Background:     Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138, is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 / SDC1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells, but only if the cells tested are already known to be derived from blood.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 50.8 kDa after removal of the signal peptide. The apparent molecular mass of CD138-hFc-His is approximately 70-100 kDa due to glycosylation.
Molecular Characterization:    CD138(Gln23-Gly254) hFc(Glu99-Ala330) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD138, hFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD138, hFc-His tagged protein (11134) can bind Anti-CD138 antibody in a linear range of 1.6-200 ng/ml.