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Human CD34 Protein, His Tag

Recombinant human CD34 protein with C-terminal 6XHis tag


Cat.#:  11280

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11280
Product Name:   Human CD34 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CD34 Molecule
Target:    CD34
UNIPROT ID:    P28906
Description:    Recombinant human CD34 protein with C-terminal 6xHis tag
Background:     The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 28.3 kDa after removal of the signal peptide.
Molecular Characterization:    CD34(Ser32-Thr290) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD34 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD34 Protein, His Tag (11280) can bind Anti-CD34 antibody(DM134), Rabbit mAb in a linear range of 0.64-3.20 ng/mL.