| Cat.#: 11280 |
| Product Name: Human CD34 Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: CD34 Molecule |
| Target: CD34 |
| UNIPROT ID: P28906 |
| Description: Recombinant human CD34 protein with C-terminal 6xHis tag |
| Background: The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 28.3 kDa after removal of the signal peptide. |
| Molecular Characterization: CD34(Ser32-Thr290) 6×His tag |
| Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human CD34 Protein, His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD34 Protein, His Tag (11280) can bind Anti-CD34 antibody(DM134), Rabbit mAb in a linear range of 0.64-3.20 ng/mL. |
|
