| Cat.#: 11241 |
| Product Name: Human CD40 Ligand Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: CD40LG;D154;D40L;IGM1;GM;MD3;-BAM;NFSF5;RAP;p39 |
| Target: CD40 Ligand |
| UNIPROT ID: P29965 |
| Description: Recombinant human CD40 Ligand Protein with N-terminal Human Fc tag |
| Background: The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 42.3 kDa after removal of the signal peptide.The apparent molecular mass of hFc-CD40 Ligand is approximately 40-53 kDa due to glycosylation. |
| Molecular Characterization: hFc(Glu99-Ala330) CD40 Ligand(Met113-Leu261) |
| Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human CD40 Ligand Protein, hFc Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD40 Ligand,hFc tagged protein (11241) can bind Human CD40, mFc-His tagged protein 11143 in a linear range of 0.51-320 ng/ml. | 
Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD40L, hFc tagged protein (11241) can bind Anti-CD40L Neutralizing antibody 28051 in a linear range of 0.64-400 ng/ml. | |
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