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Human IL-2 Superkine (Mutant, C-6His) Protein

Recombinant Human Interleukin-2 Superkine is produced by our Mammalian expression system and the target gene encoding Ala21-Thr153 (L100F, R101D, L105V, I106V, I112F) is expressed with a 6His tag at the C-terminus.
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$175.00

Cat.#:  12057

   Size:   50 μg

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          Product Description          
Cat.#:     12057
Product Name:   Human IL-2 Superkine (Mutant
Size :    10 µg, 50 µg and 100 µg
Synonyms:   Interleukin-2;IL-2;T-Cell Growth Factor;TCGF;Aldesleukin;IL2
Target:    IL-2
UNIPROT ID:    P60568
Description:    “Recombinant Human Interleukin-2 Superkine is produced by our Mammalian expression system and the target gene encoding Ala21-Thr153 (L100F
Background:     Interleukin-2(IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system,belongs to the IL-2 family. It is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions that are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine-activated killer cells, monocytes, macrophages and oligodendrocytes. New research has shown that IL-2 mutant reduced toxicity while being more potent at stimulating anti-tumor effector immune cells.
Species/Host:    HEK293
Molecular Weight:    16.5 KDa
Molecular Characterization:    Not available
Purity:    Greater than 95% as determined by reducing SDS-PAGE.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Greater than 95% as determined by reducing SDS-PAGE.

Figure 2. Loaded Human IL-2RB-Fc on Protein A Biosensor, can bind Human IL-2 Superkine with an affinity constant of 0.58 nM as determined in BLI assay.

Figure 3. Measured in a cell proliferation assay using CTLL€‘2 mouse cytotoxic T cells. The ED50 for this effect is 151.39 pg/ml.

Figure 4. Loaded Human IL-2RA-Fc on Protein A Biosensor, can bind Human IL-2 Superkine with an affinity constant of 10.4 nM as determined in BLI assay.
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