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Human LRP6 Protein, His Tag

Recombinant Human LRP6 Protein with C-terminal 6XHis tag


Cat.#:  11853

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11853
Product Name:   Human LRP6 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   ADCAD2;STHAG7
Target:    LRP6
UNIPROT ID:    O75581
Description:    Recombinant Human LRP6 Protein with C-terminal 6xHis tag
Background:     This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.[provided by RefSeq, Dec 2009]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 153.1 kDa after removal of the signal peptide. The apparent molecular mass of LRP6-His is approximately 130-250 kDa due to glycosylation.
Molecular Characterization:    LRP6(Ala20-Ala1370) 6×His tag
Purity:    The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human LRP6 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 5 µg/ml (100 µl/well) Human LRP6 Protein, His Tag (11853) can bind Human DKK1 Protein, hFc Tag(11248) in a linear range of 16-400ng/mL.