| Cat.#: 11263 |
| Product Name: Human MICA Protein |
| Size : 10 µg, 50 µg and 100 µg |
| Synonyms: MIC-A;PERB11.1 |
| Target: MICA |
| UNIPROT ID: Q29983 |
| Description: Recombinant Human MICA Protein with C-terminal 6xHis tag |
| Background: This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| Species/Host: HEK293 |
| Molecular Weight: The protein has a predicted molecular mass of 33.5 kDa after removal of the signal peptide. The apparent molecular mass of MICA-His is approximately 55-70 kDa due to glycosylation. |
| Molecular Characterization: MICA (Glu24-Trp307) 6×His tag |
| Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. |
| Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Figure 1. Human MICA Protein, His Tag on SDS-PAGE under reducing condition. | 
Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human MICA, His tagged protein (11263) can bind Human NKG2D, mFc tagged protein (11200) in a linear range of 0.064-1.6 µg/ml. | 
Figure 3. Flow cytometry analysis with 15 µg/ml Human MICA Protein, His Tag (11263) on Expi293 cells transfected with Human NKG2D protein and Human DAP10 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram). | |
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