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Human NKG2D Protein, mFc Tag

Recombinant human NKG2D protein with N-terminal mouse Fc


Cat.#:  11200

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11200
Product Name:   Human NKG2D Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   NKG2D;D314;LRK1;K cell receptor D
Target:    NKG2D
UNIPROT ID:    P26718
Description:    Recombinant human NKG2D protein with N-terminal mouse Fc
Background:     Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. This gene encodes a member of the NKG2 family. The encoded transmembrane protein is characterized by a type II membrane orientation (has an extracellular C terminus) and the presence of a C-type lectin domain. It binds to a diverse family of ligands that include MHC class I chain-related A and B proteins and UL-16 binding proteins, where ligand-receptor interactions can result in the activation of NK and T cells. The surface expression of these ligands is important for the recognition of stressed cells by the immune system, and thus this protein and its ligands are therapeutic targets for the treatment of immune diseases and cancers. Read-through transcription exists between this gene and the upstream KLRC4 (killer cell lectin-like receptor subfamily C, member 4) family member in the same cluster.
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 42.8 kDa after removal of the signal peptide.The apparent molecular mass of mFc-NKG2D is approximately 50-65 kDa due to glycosylation.
Molecular Characterization:    mFc(Pro99-Lys330) NKG2D(Ile73-Val216)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human NKG2D Protein, mFc Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human MICA, His tagged protein 11263 can bind Human NKG2D, mFc tagged protein (11200) in a linear range of 0.064-1.6 µg/ml.

Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human NKG2D, mFc tagged protein (11200) can bind Anti-NKG2D Neutralizing antibody 28048 in a linear range of 0.64-400 ng/ml.