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Human ROR1 Protein, His Tag

Recombinant Human ROR1 Protein with C-terminal 6XHis tag


Cat.#:  11267

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11267
Product Name:   Human ROR1 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   ROR1;TRKR1
Target:    ROR1
UNIPROT ID:    Q01973
Description:    Recombinant Human ROR1 Protein with C-terminal 6xHis tag
Background:     This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2012]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 42.8 kDa after removal of the signal peptide. The apparent molecular mass of ROR1-His is approximately 55-70 kDa due to glycosylation.
Molecular Characterization:    ROR1(Gln30-Glu403) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human ROR1 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human ROR1, His tagged protein  (11267) can bind Anti-ROR1 Neutralizing antibody 28076 in a linear range of 0.64-16 µg/ml.