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Human RSPO1(21-263) Protein, His Tag

Recombinant human RSPO1(21-263) protein with C-terminal 6XHis tag


Cat.#:  11567

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11567
Product Name:   Human RSPO1(21-263) Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   R-spondin-1;hRspo1
Target:    RSPO1
Description:    Recombinant human RSPO1(21-263) protein with C-terminal 6xHis tag
Background:     This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 27.6 kDa after removal of the signal peptide. The apparent molecular mass of RSPO1(21-263)-His is approximately 35-55 kDa due to glycosylation.
Molecular Characterization:    RSPO1(21-263)(Ser21-Ala263) 6×His tag
Purity:    The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human RSPO1(21-263) Protein, His Tag on SDS-PAGE under reducing condition.