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Human SPARC Protein, hFc Tag

Recombinant human SPARC protein with N-terminal human Fc tag


Cat.#:  11546

   Size:   100 μg

In Stock

          Product Description          
Cat.#:     11546
Product Name:   Human SPARC Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   BM-40;Osteonectin;ON
Target:    SPARC
UNIPROT ID:    P09486
Description:    Recombinant human SPARC protein with N-terminal human Fc tag
Background:     This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 58.8 kDa after removal of the signal peptide. The apparent molecular mass of hFc-SPARC is approximately 55-70 kDa due to glycosylation.
Molecular Characterization:    hFc(Glu99-Ala330) SPARC(Ala18-Ile303)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human SPARC Protein, hFc Tag on SDS-PAGE under reducing condition.